In vitro interaction; bait produced as a recombinant fusion protein in bacterial system; prey produced in bacterial system (prey from embryo cDNA expression library).
PI31 protein labeled by protein kinase A via the HMK tag, then used to screen a cDNA expression library.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.
