FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Prosα4, PI31
FlyBase ID
FBig0000096968
Interaction Network
Interactions Browser links
Prosα4 network
PI31 network
Reported Interactions
FBrf0221396-7.coIP.WB
Reference
Cho-Park and Steller, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Prosα4 

α4

bait

FLAG tag

PI31 

DmPI31

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cho-Park and Steller, 2013)
FBrf0221396-9.PD.WB
Reference
Cho-Park and Steller, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Prosα4 

α4

bait

FLAG tag

PI31 

DmPI31

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ADP-ribosylation of PI31 reduces its interaction with Prosα4 and decreases PI31 inhibition of the 20S proteasome in vitro.

(Cho-Park and Steller, 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293 cells; bait produced as recombinant fusion protein in bacterial system.

(Cho-Park and Steller, 2013)

PI31 was ADP-ribosylated in vitro by tankyrase.

(Cho-Park and Steller, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)