Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type ovary extract.
GRNA chromatography: grk RNA containing a BoxB sequence of the λ phage was bound to a fusion of GST with the 21 residue N antiterminator peptide.
The grk-GST particles were pre-bound to resin and incubated with ovary extract.
Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Positive control.
