Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
mTerf5 cannot bind DNA directly, but causes additional higher molecular weight forms of the mTTF-mtDNA complex. Antibodies to mTerf5 inhibit these higher weight complexes.
The interaction of mTTF and mTERF5 was assayed by the formation of complexes with a radiolabelled fragment of mitochondrial DNA spanning mt:tRNA:E and mt:tRNA:F.
