γTub37C, Grip91 Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

γTub37C, Grip91

FlyBase ID

FBig0000097102

Interaction Network

Interactions Browser links

γTub37C network
Grip91 network

Reported Interactions

FBrf0107918-9.coIP.MW

Reference

Oegema et al., 1999

Description

physical association

Assay

anti bait coimmunoprecipitation

cosedimentation through density gradient

molecular weight estimation by staining

Collection

Source/Stage

organism / embryonic stage 1 embryonic stage 8 blastoderm stage embryonic stage 5 embryonic stage 3 embryonic cycle 6 embryonic cycle 8 embryonic cycle 5 embryonic stage 6 embryonic cycle 7 embryonic cycle 4 embryonic cycle 3 embryonic stage 4 embryonic stage 7 embryonic stage 2 embryonic cycle 9

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γtub

bait

Grip91

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oegema et al., 1999)

γTub37C and l(1)dd4 coimmunoprecipitate and cosediment as part of the γTuSC (γ-tubulin small complex), or the larger γTuRC (γ-tubulin ring complex).

(Oegema et al., 1999)

FBrf0159185-18.coIP.WB

Reference

Gunawardane et al., 2003

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

bait

Grip91

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gunawardane et al., 2003)

FBrf0159185-8.coIP.MW

Reference

Gunawardane et al., 2003

Description

physical association

Assay

anti bait coimmunoprecipitation

molecular weight estimation by staining

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

bait

Grip91

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gunawardane et al., 2003)

FBrf0167865-7.coIP.WB

Reference

Kawaguchi and Zheng, 2004

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

bait

FlAG tag

Grip91

Dgrip91

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait and prey co-produced as a recombinant fusion proteins in Sf9 cell system.

(Kawaguchi and Zheng, 2004)

FBrf0201798-32.CS.WB

Reference

Wiese, 2008

Description

physical association

Assay

cosedimentation through density gradient

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γTub37CD

bait

Grip91

Dgrip91

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0219801-22.CS.WB

Reference

Gopalakrishnan et al., 2012

Description

physical association

Assay

cosedimentation through density gradient

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

neutral component

γTub37C

γ-tubulin

neutral component

γTub37C

γ-tubulin

neutral component

Grip91

neutral component

Grip91

neutral component

Grip91

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Gopalakrishnan et al., 2012)

γTub37C and l(1)dd4 co-fractionate as part of a Sas-4-containing S-γ-TuSC (γ-tubulin small) complex, or the larger S-γ-TuRC (γ-tubulin ring) complex.

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)

FBrf0244583-2.coIP.WB

Reference

Tariq et al., 2020

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-Tub

bait

GFP tag

Grip91

Dgrip91

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Tariq et al., 2020)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

Grip91,gammaTub37C

References (6)

Report Sections

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