Source was purified embryonic centrosomes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Centrosomes were isolated from highly mitotic syncytial embryos and subjected to cnn protein affinity purification.

Phosphopeptides in the affinity purified fraction were enriched by titanium dioxide affinity chromatography.

Bait mRNA was injected into unfertilized embryos where it was translated in vivo. The phosphomimetic mutations cause the bait to form scaffolds to which the prey is recruited. The ability of the scaffolds to recruit prey was taken as evidence of direct interaction.

Source was fixed embryos of transgenic fly line; bait produced from tagged injected construct; prey produced from transgenic construct.

The N-terminal region of the "Cnn-C" isoform (e.g., cnn-PA) has binding regions and regions that inhibit binding. The "Cnn-T" isoform (e.g., cnn-PD) lacks these N-terminal inhibitory regions.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic embryonic extract

The N-terminal region of the "Cnn-C" isoform (e.g., cnn-PA) has binding regions and regions that inhibit binding. The "Cnn-T" isoform (e.g., cnn-PD) lacks these N-terminal inhibitory regions