Duf/Kirre
fused to LexA DNA-binding domain
α-Actn
fused to B42 activation domain
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
kirre interacts with Actn, but not zormin or sls.
Two-hybrid system: yeast LexA-BD/B42-AD