FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(z), HDAC1
FlyBase ID
FBig0000097180
Interaction Network
Interactions Browser links
E(z) network
HDAC1 network
Reported Interactions
FBrf0132295-10.PD.WB
Reference
Tie et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait

GST tag

E(z) 
HDAC1 

RPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(z) 
N-terminal region
sufficient binding region
aa 1-73
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.

(Tie et al., 2001)
FBrf0132295-15.PD.WB
Reference
Tie et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

RPD3

bait

GST tag

E(z) 

E(Z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.

(Tie et al., 2001)
FBrf0132295-23.PD.A
Reference
Tie et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

RPD3

bait

GST tag

E(z) 

E(Z)

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Tie et al., 2001)
FBrf0132295-25.PD.A
Reference
Tie et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait

GST tag

HDAC1 

RPD3

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Tie et al., 2001)
FBrf0151814-7.CH.WB
Reference
Czermin et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(z)

neutral component
E(z) 

E(z)

neutral component
E(z) 

E(z)

neutral component
HDAC1 

RPD3

neutral component
HDAC1 

RPD3

neutral component
HDAC1 

RPD3

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous genes.

(Czermin et al., 2002)

The complex of Su(z)12, E(z), Rpd3, esc and Caf1 has an apparent size of 600-650 kDa (gel filtration).

(Czermin et al., 2002)

A histone methyltransferase activity was purified from embryonic nuclear extracts.

(Czermin et al., 2002)
FBrf0159213-10.coIP.WB
Reference
Tie et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 16blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait
HDAC1 

RPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tie et al., 2003)

This interaction of E(z) with Rpd3 is detected only in 0-16 hr embryos, but not in 18-24 hr embryos.

(Tie et al., 2003)
FBrf0159213-14.PD.WB
Reference
Tie et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 16blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

RPD3

bait

GST tag

E(z) 

E(Z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.

(Tie et al., 2003)
FBrf0159213-22.PD.A
Reference
Tie et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait

GST tag

HDAC1 

RPD3

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Tie et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)