Two-hybrid system: yeast Cytotrap. Interaction of bait and prey proteins in the cytoplasm restores the SOS/cdc25 pathway in yeast, allowing survival at elevated temperatures.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).
Source was cell extract of HEK-293T cell line; bait produced from transfected construct; prey produced from transfected construct.
