FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
insc, Gαi
FlyBase ID
FBig0000097281
Interaction Network
Interactions Browser links
insc network
Gαi network
Reported Interactions
FBrf0127308-2.coIP.MS
Reference
Schaefer et al., 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 10embryonic stage 12embryonic stage 11
Cell line used
Participants
Corresponds to
Reported as
Role
Note
insc 

Insc

bait

β-gal tag

insc 
Gαi 

Gαi

prey

2 peptides identified that map to Gαi and Gαo

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
insc 
membrane localisation domain
sufficient binding region
aa 302-459
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Schaefer et al., 2000)
FBrf0127308-8.PD.A
Reference
Schaefer et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
insc 

Insc

bait

GST tag

insc 
Gαi 

Gαi

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
insc 
central asymmetry domain
sufficient binding region
aa 252-615
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Schaefer et al., 2000)

Very weak binding.

(Schaefer et al., 2000)
FBrf0139609-1.coIP.WB
Reference
Schaefer et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
insc 

Insc

bait

β-Gal tag

insc 
Gαi 

Gαi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
insc 
asymmetry domain
sufficient binding region
aa 252-615
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

insc binds Gαi but not Gαo or Gβ13F.

(Schaefer et al., 2001)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Schaefer et al., 2001)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)