FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
rols, blow
FlyBase ID
FBig0000097339
Interaction Network
Interactions Browser links
rols network
blow network
Reported Interactions
FBrf0194627-5.Y2H
Reference
Kreiskother et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rols 

Rols7

bait

fused to LexA DNA-binding domain

rols 
rols 
blow 

Blow

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rols 
R1 fragment
sufficient binding region
aa 326-365

coordinates relative to larger \'Rols7\' isoform

rols 
TPR E repeat
sufficient binding region
aa 1815–1848

coordinates relative to larger \'Rols7\' isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kreiskother et al., 2006)

Two-hybrid system: yeast LexA-BD/B42-AD

(Kreiskother et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)