Immunoprecipitation of Nrx-1 from membrane fractions enriched for presynaptic active zone vesicles.
Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/GAL4-AD
Positive control.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; bait chemically synthesized; prey produced as a recombinant fusion protein in bacterial system.
The RhoGAP100F PDZ domain binds the Nrx-1 PDZ binding motif peptide with Kd = 5uM (ten-fold greater affinity than interaction of the Spn PDZ domain with the Nrx-1 peptide).
Positive control.
