Cell extracts were prepared from mitotic S2 cells.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

cnn may interact with γTub indirectly, as this interaction could not be verified by yeast two hybrid or other methods.

Source was purified embryonic centrosomes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Centrosomes were isolated from highly mitotic syncytial embryos and subjected to cnn protein affinity purification.

Bait mRNA was injected into unfertilized embryos where it was translated in vivo. The phosphomimetic mutations cause the bait to form scaffolds to which the prey is recruited. The ability of the scaffolds to recruit prey was taken as evidence of direct interaction.

The N-terminal region of the "Cnn-C" isoform (e.g., cnn-PA) has binding regions and regions that inhibit binding. The "Cnn-T" isoform (e.g., cnn-PD) lacks these N-terminal inhibitory region

Source was fixed embryos of transgenic fly line; bait produced from tagged injected construct; prey produced from transgenic construct.

The N-terminal region of the "Cnn-C" isoform (e.g., cnn-PA) has binding regions and regions that inhibit binding. The "Cnn-T" isoform (e.g., cnn-PD) lacks these N-terminal inhibitory regions

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.