FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pli
FlyBase ID
FBig0000097534
Interaction Network
Interactions Browser links
Pli network
Reported Interactions
FBrf0107747-4.Y2H
Reference
Großhans et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pli 

Pli

bait

fused to LexA DNA-binding domain

Pli 

Pli

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Großhans et al., 1999)

The Pli protein self-associates.

(Großhans et al., 1999)

Two-hybrid system: yeast LexA-BD/B42-AD

(Großhans et al., 1999)
FBrf0107747-7.PD.A
Reference
Großhans et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pli 

Pli

bait

GST tag

Pli 

Pli

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Pli protein self-associates.

(Großhans et al., 1999)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Großhans et al., 1999)
External Crossreferences and Linkouts ( 0 )
References (1)