FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pur-α, me31B
FlyBase ID
FBig0000097638
Interaction Network
Interactions Browser links
Pur-α network
me31B network
Reported Interactions
FBrf0219614-3.coIP.MS
Reference
Aumiller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pur-α 

Pur-α

bait

GFP tag

me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Aumiller et al., 2012)
FBrf0219614-6.coIP.WB
Reference
Aumiller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pur-α 

Pur-α

bait

GFP tag

Pur-α 
Pur-α 
Pur-α 
me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pur-α 
PUR repeats I and II
sufficient binding region
aa 40-185
Pur-α 
PUR repeats I and II
mutation disrupting interaction
aa 80-158

R80A, R158A

Pur-α 
PUR repeats I and II
mutation decreasing interaction
aa 65-142

R65A, R142A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Aumiller et al., 2012)

Mutation of the Pur-α RNA binding domain disrupts interaction with me31B protein.

(Aumiller et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)