FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Vrp1, dock
FlyBase ID
FBig0000097768
Interaction Network
Interactions Browser links
Vrp1 network
dock network
Reported Interactions
FBrf0221091-1.coIP.WB
Reference
Kaipa et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

FLAG tag

Vrp1 

Vrp1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kaipa et al., 2013)
FBrf0221091-5.Y2H
Reference
Kaipa et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vrp1 

Vrp1

bait

fused to LexA DNA-binding domain

Vrp1 
Vrp1 
dock 

Dock

prey

fused to B42 activation domain

dock 
dock 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vrp1 
proline-rich region
sufficient binding region
aa 348-635
Vrp1 
proline-rich region
necessary binding region
aa 348-635
dock 
SH3 domain 1
necessary binding region
dock 
SH3 domain 3
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Kaipa et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kaipa et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)