FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
disp, hh
FlyBase ID
FBig0000097856
Interaction Network
Interactions Browser links
disp network
hh network
Reported Interactions
FBrf0214615-1.coIP.WB
Reference
Callejo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
disp 

Disp

bait
hh 

hh

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary gland of transgenic fly line; bait produced from untagged transgenic construct; prey produced from tagged transgenic construct.

(Callejo et al., 2011)
FBrf0245833-1.MST
Reference
Cannac et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hh 

Hh

neutral component
hh 

Hh

neutral component
hh 
hh 

Hh

neutral component
hh 
disp 

Dispatched

neutral component
disp 

Dispatched

neutral component
disp 

Dispatched

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hh 
interaction domain
sufficient binding region
aa 85-257

Cys85 residue replaced with two Ile residue to mimic palmitoylation.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Kd = 6.2uM

(Cannac et al., 2020)

Interaction in vitro; hh produced as a recombinant fusion protein in bacterial system; disp purified from transfected HEK293F cells.

(Cannac et al., 2020)
FBrf0245833-2.EM
Reference
Cannac et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hh 

Hh

neutral component
hh 

Hh

neutral component
hh 
hh 

Hh

neutral component
hh 
disp 

Dispatched

neutral component
disp 

Dispatched

neutral component
disp 

Dispatched

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hh 
interaction domain
sufficient binding region
aa 85-257

Cys85 residue replaced with two Ile residue to mimic palmitoylation.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; hh produced as a recombinant fusion protein in bacterial system; disp purified from transfected HEK293F cells.

(Cannac et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)