Source was adult ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

In the presence of RNase during immunoprecipitation this interaction was not observed, indicating that it is probably indirect and mediated through RNA in the RNP complex.

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Treatment with RNase weakens the me31B-cup interaction.

In the presence of RNase during immunoprecipitation this interaction is still observed, indicating that it is not mediated through RNA in the RNP complex; may be direct.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

cup was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

Source was live S2R+ cells; bait and prey produced from endogenous genes.