FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
WASp, dock
FlyBase ID
FBig0000097954
Interaction Network
Interactions Browser links
WASp network
dock network
Reported Interactions
FBrf0144932-10.Y2H
Reference
Worby et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

fused to LexA DNA-binding domain

WASp 

Wasp

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Worby et al., 2001)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Worby et al., 2001)
FBrf0221091-2.coIP.WB
Reference
Kaipa et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

FLAG tag

WASp 

WASp

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kaipa et al., 2013)
FBrf0221091-3.coIP.WB
Reference
Kaipa et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASp

bait

HA tag

dock 

Dock

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kaipa et al., 2013)
FBrf0221091-6.Y2H
Reference
Kaipa et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASp

bait

fused to LexA DNA-binding domain

WASp 
WASp 
dock 

Dock

prey

fused to B42 activation domain

dock 
dock 
dock 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
WASp 
proline-rich region
sufficient binding region
aa 310-415
WASp 
proline-rich region
necessary binding region
aa 310-415
dock 
SH3 domain 1
necessary binding region
dock 
SH3 domain 2
necessary binding region
dock 
SH3 domain 3
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Kaipa et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kaipa et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)