γTub37C, Grip128 Physical Interaction report

cosedimentation through density gradient

Collection

Source/Stage

organism / embryonic stage 1 embryonic stage 8 blastoderm stage embryonic stage 5 embryonic stage 3 embryonic cycle 6 embryonic cycle 8 embryonic cycle 5 embryonic stage 6 embryonic cycle 7 embryonic cycle 4 embryonic cycle 3 embryonic stage 4 embryonic stage 7 embryonic stage 2 embryonic cycle 9

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γtub

bait

Grip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oegema et al., 1999)

γTub37C and Grip128 coimmunoprecipitate and cosediment as part of the γTuRC (γ-tubulin ring complex).

(Oegema et al., 1999)

FBrf0159185-17.coIP.WB

Reference

Gunawardane et al., 2003

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

bait

Grip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gunawardane et al., 2003)

FBrf0159185-50.coIP.MW

Reference

Gunawardane et al., 2003

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γtubulin

bait

Grip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)

FBrf0159185-51.coIP.MW

Reference

Gunawardane et al., 2003

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Grip128

bait

γTub37C

γtubulin

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)

FBrf0159185-54.coIP.WB

Reference

Gunawardane et al., 2003

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γtubulin

bait

Grip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)

FBrf0159185-55.coIP.WB

Reference

Gunawardane et al., 2003

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Grip128

bait

γTub37C

γtubulin

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)

FBrf0159185-7.coIP.MW

Reference

Gunawardane et al., 2003

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

bait

Grip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gunawardane et al., 2003)

FBrf0201798-29.CoIP.WB

Reference

Wiese, 2008

Description

physical association

Assay

cosedimentation through density gradient

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γTub37CD

bait

Grip128

Dgrip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly linee; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0201798-33.CS.WB

Reference

Wiese, 2008

Description

physical association

Assay

cosedimentation through density gradient

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γTub37CD

bait

Grip128

Dgrip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0219801-33.CS.WB

Reference

Gopalakrishnan et al., 2012

Description

physical association

Assay

anti tag coimmunoprecipitation

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub37C

γ-tubulin

neutral component

γTub37C

γ-tubulin

neutral component

γTub37C

γ-tubulin

neutral component

Grip128

neutral component

Grip128

neutral component

Grip128

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Gopalakrishnan et al., 2012)

γTub37C and Grip128 co-fractionate as part of a Sas-4-containing S-γ-TuRC (γ-tubulin ring) complex.

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)

FBrf0244583-10.coIP.MW

Reference

Tariq et al., 2020

Description

physical association

Assay