FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
mle, msl-2
FlyBase ID
FBig0000098130
Interaction Network
Interactions Browser links
mle network
msl-2 network
Reported Interactions
FBrf0104740-8.coIP.WB
Reference
Copps et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE1

bait
msl-2 

MSL2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Copps et al., 1998)

Weak coimmunoprecipitation of MSL proteins compared to the amount of mle immunoprecipitated, suggesting that only a fraction of mle is associated with MSL proteins.

(Copps et al., 1998)
FBrf0204458-2.coIP.WB
Reference
Li et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-2 

MSL2

bait

FLAG tag

msl-2 
mle 

MLE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-2 
C-terminal domain
sufficient binding region
aa 490-773
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult females of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2008)
FBrf0204458-3.coIP.WB
Reference
Li et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-2 

MSL2

bait
mle 

MLE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild-type adult female flies; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2008)
FBrf0222160-9a.coIP.WB
Reference
Maenner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait

FLAG tag

msl-2 

MSL2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system.

(Maenner et al., 2013)
FBrf0229405-11.PLA
Reference
Lindehell et al., 2015
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-2 

msl-2

neutral component
msl-2 

msl-2

neutral component
msl-2 

msl-2

neutral component
mle 

mle

neutral component
mle 

mle

neutral component
mle 

mle

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

(Lindehell et al., 2015)

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

(Lindehell et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)