FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Abd-B, exd
FlyBase ID
FBig0000098232
Interaction Network
Interactions Browser links
Abd-B network
exd network
Reported Interactions
FBrf0218732-13.EMSA.A
Reference
Hudry et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

Exd

neutral component
exd 

Exd

neutral component
exd 

Exd

neutral component
Abd-B 

AbdB

neutral component
Abd-B 

AbdB

neutral component
Abd-B 

AbdB

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced by in vitro transcription/translation.

(Hudry et al., 2012)

exd, hth and Abd-B, in various combinations, were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA, corresponding to Abd-B-exd-hth heterotrimers, were used to deduce protein-protein interactions.

(Hudry et al., 2012)
FBrf0218732-6.BiFC.FM
Reference
Hudry et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

Exd

bait

Venus tag, N-terminal fragment

exd 
Abd-B 

AbdB

prey

Venus tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
exd 
HD domain residue 51
mutation decreasing interaction
aa 51

N51A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Hudry et al., 2012)
FBrf0221821-1.PD.A
Reference
Rivas et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abd-B 

AbdB

bait

GST tag

Abd-B 
exd 

Exd

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abd-B 
homeodomain
sufficient binding region
aa 386-446
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Rivas et al., 2013)
FBrf0243360-1.BiFC.FM
Reference
Romero-Pozuelo et al., 2019
Description
physical association
Assay
Collection
Source/Stage
genital disc / femalelarval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

Exd

bait

YFP tag, N-terminal fragment

Abd-B 

Abd-BR

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was female genital discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Romero-Pozuelo et al., 2019)
FBrf0243360-6.BiFC.FM
Reference
Romero-Pozuelo et al., 2019
Description
physical association
Assay
Collection
Source/Stage
wing disc / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

Exd

bait

YFP tag, N-terminal fragment

Abd-B 

Abd-BR

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Romero-Pozuelo et al., 2019)
FBrf0261504-3.BiFC.FM
Reference
Curt et al., 2025
Description
physical association
Assay
Collection
Source/Stage
pupal abdomen / pupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

exd

bait

Venus tag, N-terminal fragment

Abd-B 

Abd-B

prey

Venus tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was dorsal pupal abdomens; proteins produced from transgenic constructs.

(Curt et al., 2025)
FBrf0261504-5.EMSA.A
Reference
Curt et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abd-B 

Abd-B

neutral component
Abd-B 
Abd-B 
Abd-B 

Abd-B

neutral component
Abd-B 
Abd-B 
Abd-B 

Abd-B

neutral component
exd 

exd

neutral component
exd 

exd

neutral component
exd 

exd

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abd-B 
HX-like motif residue 158
mutation decreasing interaction
aa 158

coordinates W158A

Abd-B 
C-terminal interaction residues 224 and 225
mutation decreasing interaction
aa 224-225

coordinates QR224-225AA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Abd-B, hth and exd form a trimeric complex on [32]P labeled DNA. HX-like motif and C-terminal mutations in Abd-B affect binding to CycE DNA but not to Dll DNA.

(Curt et al., 2025)

Interaction in vitro; proteins produced by in vitro translation.

(Curt et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)