FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gug, ft
FlyBase ID
FBig0000098333
Interaction Network
Interactions Browser links
Gug network
ft network
Reported Interactions
FBrf0158896-1.Y2H
Reference
Fanto et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Fat

bait

fused to GAL4 DNA-binding domain

ft 
Gug 

Atro

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
cytoplasmic domain
sufficient binding region
aa 4921-5070

NcoI-BsrBI cDNA fragment

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Fanto et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Fanto et al., 2003)
FBrf0158896-2.PD.WB
Reference
Fanto et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

GST tag

ft 

Fat

prey

FLAG tag

ft 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
cytoplasmic domain
sufficient binding region
aa 4921-5070

NcoI-BsrBI cDNA fragment

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Fanto et al., 2003)
FBrf0226170-5.PD.WB
Reference
Sing et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Ft

bait

GST tag

ft 
Gug 

Atro

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
intracellular domain
sufficient binding region

"IC" construct, coordinates not reported

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sing et al., 2014)

Positive control.

(Sing et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)