FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Aplip1, Appl
FlyBase ID
FBig0000098364
Interaction Network
Interactions Browser links
Aplip1 network
Appl network
Reported Interactions
FBrf0149603-1.Y2H
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait

fused to GAL4 DNA-binding domain

Appl 
Aplip1 

APLIP1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Appl 
cytoplasmic domain
sufficient binding region
aa 834-886
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library).

(Taru et al., 2002)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Taru et al., 2002)
FBrf0149603-2.PD.WB
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Aplip1 

APLIP1

bait

GST tag

Appl 

APPL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected COS-7 cell extract.

(Taru et al., 2002)
FBrf0149603-3.coIP.WB
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Aplip1 

APLIP1

bait

FLAG tag

Appl 

APPL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Taru et al., 2002)
FBrf0149603-4.coIP.WB
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait
Aplip1 

APLIP1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Taru et al., 2002)
FBrf0149603-5.Y2H
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait

fused to GAL4 DNA-binding domain

Appl 
Appl 
Aplip1 

APLIP1

prey

fused to GAL4 activation domain

Aplip1 
Aplip1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Appl 
cytoplasmic domain
sufficient binding region
aa 855-886

\'NΔ21\'

Appl 
cytoplasmic domain
necessary binding region
aa 873-882

\'Δ873-882\'

Aplip1 
C-terminal region
sufficient binding region
aa 208-490

\'NΔ207\'

Aplip1 
C-terminal region
necessary binding region
aa 354-490

\'CΔ137\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Taru et al., 2002)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Taru et al., 2002)
FBrf0149603-6.PD.WB
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait

GST tag

Appl 
Appl 
Aplip1 

APLIP1

prey

Myc tag

Aplip1 
Aplip1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Appl 
cytoplasmic domain
sufficient binding region
aa 834-886

\'APPLcyt\'

Appl 
cytoplasmic domain
necessary binding region
aa 873-882

\'Δ873-882\'

Aplip1 
C-terminal region
sufficient binding region
aa 208-490

\'NΔ207\'

Aplip1 
C-terminal region
necessary binding region
aa 354-490

\'CΔ137\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected COS-7 cell extract.

(Taru et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)