FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098461
Interaction Network
Interactions Browser links
capu network
Reported Interactions
FBrf0191267-4.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
capu 

CapuN3

bait

GST tag

capu 

CapuC2

prey

[35]S label

capu 
capu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
capu 
C-terminal region
sufficient binding region
aa 900-1058

\'C2\'

capu 
Rho-binding domain
sufficient binding region
aa 125-250

\'N3\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

capu exhibits intramolecular interaction between N- and C- terminal regions.

FBrf0191267-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
capu 

CapuFH2

bait

GST tag

capu 

CapuFH2

prey

[35]S label

capu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
capu 
FH2 domain
sufficient binding region
aa 584-1058
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

The capu FH2 domain homodimerizes.

FBrf0219599-1.FPS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
capu 

capu

bait

GST tag

capu 

capu

prey

Alexa 488 label

capu 
capu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
capu 
Tail domain
sufficient binding region
aa 467-1047
capu 
N-terminal domain
sufficient binding region
aa 1-222
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Alexa 488 labeled capu tail domain was incubated with increasing amounts of capu N-terminal regions. capu N-terminal aa 1-321 displayed equal binding affinty to aa 1-466 with a Kd of 300nM.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system and labeled in vitro.

FBrf0219599-2.CH.SLS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
capu 

capu

self
capu 

capu

self
capu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
capu 
N-terminal dimerization domain
sufficient binding region
aa 222-321
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

capu N-terminal domain forms a dimer.

External Crossreferences and Linkouts ( 0 )
References (2)