FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lam
FlyBase ID
FBig0000098732
Interaction Network
Interactions Browser links
Lam network
Reported Interactions
FBrf0102927-4.Y2H
Reference
Goldberg et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lamin

bait

fused to GAL4 DNA-binding domain

Lam 

Lamin

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Goldberg et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Goldberg et al., 1998)
FBrf0109097-2.Y2H
Reference
Stuurman et al., 1999
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lam 

lamin

bait

fused to GAL4 DNA-binding domain

Lam 

lamin

prey

fused to GAL4 activation domain

Lam 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lam 
interaction domain
sufficient binding region
aa 57-542
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Stuurman et al., 1999)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Stuurman et al., 1999)
FBrf0208977-2.Y2H
Reference
Schulze et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lam Dm0

bait

fused to GAL4 DNA-binding domain

Lam 

Lam Dm0

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Schulze et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Schulze et al., 2009)
FBrf0217598-1.CS.WB
Reference
Zaremba-Czogalla et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lam 

Lam

neutral component
Lam 

Lam

neutral component
Lam 
Lam 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lam 
region residue 64
mutation decreasing interaction
aa 64

R64H

Lam 
phosphosite residue 435
mutation decreasing interaction
aa 435

T435E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Relative amounts of protein in pellet vs soluble fractions taken by author as measure of polymerization.

(Zaremba-Czogalla et al., 2012)

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Zaremba-Czogalla et al., 2012)
External Crossreferences and Linkouts ( 0 )
References (4)