FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nwk, WASp
FlyBase ID
FBig0000098874
Interaction Network
Interactions Browser links
nwk network
WASp network
Reported Interactions
FBrf0175021-1.PD.WB
Reference
Coyle et al., 2004
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

GST tag

nwk 
WASp 

Wsp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
SH3a domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Coyle et al., 2004)
FBrf0175021-2.Y2H
Reference
Coyle et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

fused to GAL4 DNA-binding domain

nwk 
WASp 

Wsp

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Coyle et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Coyle et al., 2004)
FBrf0205746-3.PD.WB
Reference
Rodal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

GST tag

nwk 
nwk 
WASp 

Wsp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
N-terminal region
sufficient binding region
aa 1-754

coordinates relative to nwk-PB

nwk 
SH3a domain residue W531
mutation disrupting interaction
aa 531

W531A, coordinates relative to nwk-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Rodal et al., 2008)
FBrf0205746-8.ENZ
Reference
Rodal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
WASp 

Wsp

ancillary

His tag

WASp 

Wsp

ancillary

His tag

WASp 

Wsp

ancillary

His tag

nwk 

Nwk

ancillary

His tag

nwk 
nwk 

Nwk

ancillary

His tag

nwk 

Nwk

ancillary

His tag

nwk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
N-terminal region
sufficient binding region
aa 1-754

coordinates relative to nwk-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Rodal et al., 2008)

Bovine Arp2/3 complex was mixed with pyrene labeled actin, to which WASp, nwk and Cdc42 were added in various combinations. Actin polymerization was monitored by fluorescence.

(Rodal et al., 2008)
FBrf0233532-1.PD.WB
Reference
Stanishneva-Konovalova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nwk 

Nwk

bait

GST tag

nwk 
WASp 

WASp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nwk 
SH3ab domain
sufficient binding region
aa 547-696

coordinates relative to nwk-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Stanishneva-Konovalova et al., 2016)

The nwk F-BAR domain (aa 1-428) competes with WASp for binding to the nwk SH3ab domain.

(Stanishneva-Konovalova et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)