BicD
GFP tag
Dhc
L731A, disrupts cargo binding too
F684I, increases binding to Dynein motor
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
In double BicD mutants, the L731A BicD mutation, which disrupts cargo-binding to BicD, also prevents increased binding to Dhc64C caused by the F684I mutation.
