FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
veli, dlg1
FlyBase ID
FBig0000098959
Interaction Network
Interactions Browser links
veli network
dlg1 network
Reported Interactions
FBrf0174808-2.Y2H
Reference
Bachmann et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
veli 

DLin-7

bait

fused to GAL4 DNA-binding domain

dlg1 

Dlg-S97

prey

fused to GAL4 activation domain

dlg1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dlg1 
L27 domain
sufficient binding region
aa 1-150

998 residue \'S97\' isoform tested

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bachmann et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bachmann et al., 2004)

veli binds sdt and CASK more strongly than dlg1 in this assay.

(Bachmann et al., 2004)
FBrf0174808-6.coIP.WB
Reference
Bachmann et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
veli 

Lin-7

bait

FLAG tag

dlg1 

Dlg-S97

prey
dlg1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bachmann et al., 2004)
FBrf0222215-1.coIP.WB
Reference
Soukup et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dlg1 

DLG

bait
veli 

Dlin7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Soukup et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)