FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Khc-73, dlg1
FlyBase ID
FBig0000099009
Interaction Network
Interactions Browser links
Khc-73 network
dlg1 network
Reported Interactions
FBrf0189924-2.coIP.WB
Reference
Siegrist and Doe, 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc-73 

Khc-73

bait

HA tag

Khc-73 
dlg1 

Dlg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc-73 
MBS domain
sufficient binding region
aa 581-800
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Siegrist and Doe, 2005)

The dlg1-Khc-73 interaction could not by detected using in vitro binding assays.

(Siegrist and Doe, 2005)
FBrf0223553-1.PD.MW
Reference
Lu and Prehoda, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dlg1 

Dlg

bait

GST tag

dlg1 
Khc-73 

Khc73

prey

His tag

Khc-73 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dlg1 
GK domain
sufficient binding region
aa 771-975

coordinates relative to dlg1-PG

Khc-73 
MBS domain
sufficient binding region
aa 580-829

coordinates relative to Khc-73-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lu and Prehoda, 2013)
FBrf0237390-2.PD.WB
Reference
Golub et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc-73 

Khc73

bait

GST tag

Khc-73 
dlg1 

Dlg

prey

His tag

dlg1 
dlg1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc-73 
MBS domain
sufficient binding region
aa unspecified
dlg1 
GK domain
sufficient binding region
aa unspecified
dlg1 
SH3GK domain
sufficient binding region
aa unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Golub et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)