grk
SA tag
BicD
Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.
BicD and egl were the only proteins pulled down consistently and specifically with wild-type localization signals from fs(1)K10 (TLS), grk (GLS), h (SL1) and I-element (ILS).
Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.