Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).
Though CLIP-190 binds apical RNA localization signals specifically, it does not appear to be required for apical RNA transport.
Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.
Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).
Though CLIP-190 binds apical RNA localization signals specifically, it does not appear to be required for apical RNA transport.
Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.