FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trp
FlyBase ID
FBig0000099226
Interaction Network
Interactions Browser links
trp network
Reported Interactions
FBrf0097895-12.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait
trp 

TRP

prey

MYC tag

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-14.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait

MYC tag

trp 

TRP

prey

Flag tag

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-16.coIP.WB
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait
trp 

TRP

prey

MYC tag

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trp 
TM domain residues 324-672
sufficient binding region
aa 324-672
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of a 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1997)
FBrf0097895-6.Y2H
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait

fused to GAL4 DNA-binding domain

trp 

TRP

prey

fused to GAL4 activation domain

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-338
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Xu et al., 1997)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Xu et al., 1997)
FBrf0097895-9.PD.A
Reference
Xu et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait

GST tag

trp 

TRP

prey

[35]S label

trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trp 
N-terminal interaction domain residues 1-333
sufficient binding region
aa 1-333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Xu et al., 1997)
FBrf0222115-1.coIP.WB
Reference
Katz et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

TRP

bait

GFP tag

trp 

TRP

prey
trp 
trp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trp 
C-terminal region
necessary binding region
aa 675-1275
trp 
N-terminal region
necessary binding region
aa 1-328
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic trp expressed in photoreceptor cells. Anti-GFP antibody used for immunoprecipitation.

(Katz et al., 2013)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Katz et al., 2013)

trp forms homomultimers. trp does not interact with trpl.

(Katz et al., 2013)

In trp::trpl chimeric proteins, a trp derived C-terminal intracellular region is necessary for interaction with endogenous trp. A trp-derived N-terminal intracellular region is required to prevent formation of trp-trpl heteromultimers.

(Katz et al., 2013)
FBrf0235287-2.coIP.WB
Reference
Voolstra et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trp 

trp

bait

GFP tag

trp 

trp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Voolstra et al., 2017)
External Crossreferences and Linkouts ( 0 )
References (3)