Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was body wall muscle of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The amount of Fas2 that was coimmunoprecipitated was dependent om rl activity; reduced rl activity resulted in an increase of Fas2, while increased rl activity resulted in a decrease of Fas2 coimmunoprecipitated.