FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sce, RAF2
FlyBase ID
FBig0000099313
Interaction Network
Interactions Browser links
Sce network
RAF2 network
Reported Interactions
FBrf0215737-10.coIP.MS
Reference
Lagarou et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sce 

dRING

bait
RAF2 

dRAF2

prey

17 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lagarou et al., 2008)

Sce, Psc, Mtor, Ulp1, Kdm2 and RAF2 constitute the dRAF complex.

(Lagarou et al., 2008)

Nuclear extract was partially purified by ammonium sulphate precipitation and heparin affinity chromatography, then depleted of Pc-containing complexes before use in immunoprecipitation.

(Lagarou et al., 2008)
FBrf0215737-25.coIP.WB
Reference
Lagarou et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sce 

dRING

bait
RAF2 

dRAF2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lagarou et al., 2008)
FBrf0215737-28.coIP.WB
Reference
Lagarou et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RAF2 

dRAF2

bait
Sce 

dRING

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lagarou et al., 2008)

RAF2 coimmunoprecipitates Sce and Psc, but not Pc or ph-p/ph-d.

(Lagarou et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)