FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Egfr, Egfrap
FlyBase ID
FBig0000099459
Interaction Network
Interactions Browser links
Egfr network
Egfrap network
Reported Interactions
FBrf0222949-21.Y2H
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Egfr 

EGFR

bait
Egfr 
Egfr 
Egfrap 

CG13290

prey
Egfrap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Egfr 
intracellular domain
sufficient binding region
Egfr 
intracellular domain
mutation decreasing interaction
aa 1095-1342

all Tyr residues mutated to Phe in this region

Egfrap 
SH2 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

(Bianco et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bianco et al., 2007)
FBrf0250898-1.Y2H
Reference
Soler Beatty et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Egfr 

Egfr

bait

fused to GAL4 DNA-binding domain

Egfr 
Egfrap 

Egfrap

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Egfr 
cytoplasmic tail domain residue 1357
necessary binding region
aa 1357

Y1357F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Soler Beatty et al., 2021)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Soler Beatty et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)