FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4E7, mxt
FlyBase ID
FBig0000099591
Interaction Network
Interactions Browser links
eIF4E7 network
mxt network
Reported Interactions
FBrf0222078-6.Y2H
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E7 

eIF4E-7

bait

fused to GAL4 DNA-binding domain

mxt 

Mxt

prey

fused to GAL4 activation domain

mxt 
mxt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
C-terminal region
sufficient binding region
aa 284-653
mxt 
eIF4E-binding motif
mutation disrupting interaction
YDIEHLL

\'Mxt[AAA]\', Y581A, L586A, L587A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hernández et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hernández et al., 2013)

CG2950 (mxt) binds eIF4E, eIF4E-3, eIF4E-4 and eIF4E-7, but not eIF4E-6 or 4EHP in this assay.

(Hernández et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)