FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
14-3-3ζ, 14-3-3ε
FlyBase ID
FBig0000100586
Interaction Network
Interactions Browser links
14-3-3ζ network
14-3-3ε network
Reported Interactions
FBrf0209680-2.CL.WB
Reference
Messaritou et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

neutral component
14-3-3ζ 

14-3-3ζ

neutral component
14-3-3ζ 

14-3-3ζ

neutral component
14-3-3ε 

14-3-3ε

neutral component
14-3-3ε 

14-3-3ε

neutral component
14-3-3ε 

14-3-3ε

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Messaritou et al., 2010)
FBrf0209680-5.coIP.WB
Reference
Messaritou et al., 2010
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

FLAG tag

14-3-3ζ 
14-3-3ε 

14-3-3ε

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
14-3-3ζ 
Arg Val Glu and Arg Val Glu interaction domain
mutation disrupting interaction
aa 15-17,88-90

15LAE17 to 15QQR17 and 88AVE90 to 88NVQ90

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Messaritou et al., 2010)
FBrf0223553-4.PD.WB
Reference
Lu and Prehoda, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

GST tag

14-3-3ε 

14-3-3ε

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lu and Prehoda, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)