FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Khc-73, 14-3-3ε
FlyBase ID
FBig0000100656
Interaction Network
Interactions Browser links
Khc-73 network
14-3-3ε network
Reported Interactions
FBrf0223553-6.coIP.WB
Reference
Lu and Prehoda, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc-73 

Khc73

bait

Myc tag

Khc-73 
14-3-3ε 

14-3-3ε

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc-73 
14-3-3 binding motif
mutation disrupting interaction
aa 1374

S1374A, blocks phosphorylation, coordinates relative to Khc-73-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lu and Prehoda, 2013)

Khc-73, nudE, 14-3-3ζ and 14-3-3ε form a quaternary complex, with a 14-3-3 heterodimer bridging Khc-73 and nudE.

(Lu and Prehoda, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)