FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
FLASH, Cpsf73
FlyBase ID
FBig0000100987
Interaction Network
Interactions Browser links
FLASH network
Cpsf73 network
Reported Interactions
FBrf0223323-12.coIP.WB
Reference
Sabath et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
FLASH 

FLASH

bait
Cpsf73 

CPSF73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sabath et al., 2013)
FBrf0223323-13.coIP.MS
Reference
Sabath et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
FLASH 

FLASH

bait
Cpsf73 

CPSF73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sabath et al., 2013)
FBrf0223323-6.PD.WB
Reference
Sabath et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
FLASH 

FLASH

bait

GST tag

FLASH 
FLASH 
FLASH 
Cpsf73 

CPSF73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
FLASH 
N-terminal region
sufficient binding region
aa 1-178

\'FN-ter\'

FLASH 
N-terminal region
necessary binding region
aa 1-77

\'Δ77\'

FLASH 
LDIY motif
mutation disrupting interaction
aa 45-48

LDIY to AAAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from Kc cell nuclear extract.

(Sabath et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)