dPNUTS
RNAPII
\'dPNUTS-S\', ΔC-terminus
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Deletion of the PNUTS PP1-binding motif increases the coimmunoprecipitation of hyper-phosphorylated RpII215 (RNAPIIo), but does not affect the interaction of PNUTS with hypophosphorylated RpII215 (RNAPIIa).
Myc tag
W726A
Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Ser5-phosphorylated RpII215 (RNAPII Ser5-P) coimmunoprecipitates with PNUTS if the PP1-binding motif is mutated, but not with wild-type PNUTS.
