FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lwr, tna
FlyBase ID
FBig0000101646
Interaction Network
Interactions Browser links
lwr network
tna network
Reported Interactions
FBrf0221393-1.Y2H
Reference
Monribot-Villanueva et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tna 

TnaA

bait

fused to GAL4 DNA-binding domain

tna 
lwr 

Ubc9

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tna 
XSPRING domain
sufficient binding region
aa 379-927

\'XSPRING2\' construct, coordinates relative to 1109aa isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Monribot-Villanueva et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Monribot-Villanueva et al., 2013)
FBrf0221393-3.PD.WB
Reference
Monribot-Villanueva et al., 2013
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 7embryonic stage 17late extended germ band stageembryonic stage 12embryonic stage 11embryonic stage 15embryonic stage 8dorsal closure stageembryonic stage 9early extended germ band stageembryonic stage 13embryonic stage 14embryonic stage 10extended germ band stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lwr 

Ubc9

bait

GST tag

tna 

TnaA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.

(Monribot-Villanueva et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)