Interaction in vitro; bait and prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Binding enhanced by the addition of Ca2+. Syt1 mutation D414N,D416N abolishes enhanced binding with Ca2+.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult male head extract.
Syt1 multimerization occurred in the absence of Ca[2+], but was greatly enhanced in the presence of 1 mM or 10mM Ca[2+].
Mutation of C2 domain Asp residues involved in the binding of Ca[2+] did not disrupt the Ca[2+] enhanced multimerization of Syt1 : D282N and D284N in the C2A domain, and D416N and D418N in the C2B domain.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Syt1 multimerization occurred in the absence of Ca[2+], but was greatly enhanced in the presence of 1 mM or 10mM Ca[2+].
Mutation of C2 domain Asp residues involved in the binding of Ca[2+] did not disrupt the Ca[2+] enhanced multimerization of Syt1 : D282N and D284N in the C2A domain, and D416N and D418N in the C2B domain.
