FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Kap-α3, N
FlyBase ID
FBig0000101773
Interaction Network
Interactions Browser links
Kap-α3 network
N network
Reported Interactions
FBrf0222099-1.Y2H
Reference
Sachan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

A

bait

fused to LexA DNA-binding domain

N 
Kap-α3 

Importin-α3

prey

fused to GAL4 activation domain

Kap-α3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1765-1895
Kap-α3 
C-terminal region
sufficient binding region
aa 240-502
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Sachan et al., 2013)

21 overlapping Kap-α3 clones recovered using N two-hybrid bait.

(Sachan et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Sachan et al., 2013)
FBrf0222099-3.PD.WB
Reference
Sachan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap-α3 

Imp-α3

bait

GST tag

Kap-α3 
N 

N

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Kap-α3 
C-terminal region
sufficient binding region
aa 225-514
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type third instar larval salivary gland extract.

(Sachan et al., 2013)
FBrf0222099-4.coIP.WB
Reference
Sachan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap-α3 

Imp

bait

HA tag

N 

N

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary glands of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sachan et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)