AbdA
Venus tag, N-terminal fragment
Med19
Venus tag, C-terminal fragment
Source was fixed embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
Using this assay, the abd-A-MED19 interaction is observed only with an abd-A homeodomain construct, and not for full length abd-A.
Abd-A
GST tag
[35]S label
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.