FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spag, Nop56
FlyBase ID
FBig0000102206
Interaction Network
Interactions Browser links
spag network
Nop56 network
Reported Interactions
FBrf0224244-2.Y2H
Reference
Benbahouche et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spag 

spag

bait

fused to GAL4 DNA-binding domain

spag 
Nop56 

CG13849

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spag 
TPR motifs
necessary binding region
aa 96-197
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo or ovary cDNA expression library).

(Benbahouche et al., 2014)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Benbahouche et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)