FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Orco, Or43a
FlyBase ID
FBig0000102316
Interaction Network
Interactions Browser links
Orco network
Or43a network
Reported Interactions
FBrf0184196-3.BRET
Reference
Neuhaus et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orco 

DOR83b

bait

Luciferase tag

Or43a 

DOR43a

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live HEK293 cells; bait produced from transfected construct; prey produced from transfected construct.

(Neuhaus et al., 2005)
FBrf0192657-2.FC
Reference
Benton et al., 2006
Description
physical association
Assay
Collection
Source/Stage
antenna / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Or43a 

OR43a

bait

YFP tag, N-terminal fragment

Orco 

OR83b

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Benton et al., 2006)
FBrf0192657-4.Y2H
Reference
Benton et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orco 

OR83b

bait

fused to GAL4 DNA-binding domain

Orco 
Or43a 

OR43a

prey

fused to GAL4 activation domain

Or43a 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Orco 
intercellular loop 3
sufficient binding region
aa 412-459
Or43a 
intercellular loop 3
sufficient binding region
aa 298-342
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Benton et al., 2006)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Benton et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)