Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Proteins were separately purified, mixed and refolded. Complex formation was examined using gel filtration.
W22R, coordinates relative to cal1-PA
W22R,F29A or W22R,F29R coordinates relative to cal1-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.