FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102400
Interaction Network
Interactions Browser links
cal1 network
His4 network
Reported Interactions
FBrf0223977-4.CH
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

neutral component
cal1 

CAL1

neutral component
cal1 
cal1 

CAL1

neutral component
cal1 
His4 

H4

neutral component
His4 

H4

neutral component
His4 

H4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cal1 
N-terminal region
sufficient binding region
aa 1-160
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

cid, His4 and cal1 form a complex.

Proteins were separately purified, mixed and refolded. Complex formation was examined using gel filtration.

FBrf0245264-11.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

bait

His tag

cal1 
cal1 
cal1 
His4 

H4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cal1 
N-terminal domain
sufficient binding region
aa 1-160
cal1 
interaction domain residue 22
mutation decreasing interaction
aa 22

W22R, coordinates relative to cal1-PA

cal1 
interaction domain residues 22,29
mutation disrupting interaction
aa 22,29

W22R,F29A or W22R,F29R coordinates relative to cal1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

cal1 was used as bait to pull down a cid-His4 complex.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0245264-2.CH.SLS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

neutral component
cal1 
cal1 

CAL1

neutral component
cal1 
cal1 

CAL1

neutral component
His4 

H4

neutral component
His4 

H4

neutral component
His4 

H4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cal1 
N-terminal domain
sufficient binding region
aa 1-50
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Trimeric complex consisting of cal1, cid and His4 was captured as three binary interactions.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0245264-5.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

neutral component
cal1 

CAL1

neutral component
cal1 
cal1 

CAL1

neutral component
cal1 
His4 

H4

neutral component
His4 

H4

neutral component
His4 

H4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cal1 
N-terminal domain
sufficient binding region
aa 1-160
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Trimeric complex consisting of cal1, cid and His4 was captured as three binary interactions.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0245264-8.CL.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cal1 

CAL1

neutral component
cal1 
cal1 

CAL1

neutral component
cal1 
cal1 

CAL1

neutral component
His4 

H4

neutral component
His4 

H4

neutral component
His4 

H4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cal1 
N-terminal domain
sufficient binding region
aa 1-160
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Trimeric complex consisting of cal1, cid and His4 was captured as three binary interactions.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 0 )
References (2)