Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

TAP-tagged bait protein added to an in vitro splicing reaction which included embryonic nuclear extract and [32]P labeled prey RNA. tsu and mago produced as a fusion protein, captured as two separate interactions.

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Overexpression of Pym (either full length or residues 1-152) causes the dissociation of mago (an EJC component) from osk RNA.