Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Only those co-purified proteins identified in all three replicates, and not in negative controls, were reported.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

MBP-tagged sstn was fractionated by SDS-PAGE, transferred to nitrocellulose, then overlaid with GST-tagged step. Binding of step to sstn was assessed by anti-GST antibodies.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Only those co-purified proteins identified in all three replicates, and not in negative controls, were reported.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Co-expression of tagged step with tagged sstn increases the amount of step copurifying with sstn, and decreases the co-purification of other proteins with sstn.

Only those co-purified proteins identified in all three replicates, and not in negative controls, were reported.